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Therefore, further studies with fresh T1D patient-derived PBMCs must be performed to validate these findings and uncover any new insights

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This low-grade systemic inflammation: Impairs insulin receptor signalling, directly worsening insulin resistance Damages ovarian granulosa cells, impairing egg development and ovarian function Disrupts gut barrier integrity, contributing to what some researchers call a "leaky gut" pattern associated with systemic inflammation Amplifies HPA axis dysregulation, raising cortisol and further disrupting sex hormone balance Chronic inflammation is both a consequence of PMOS and a driver that perpetuates it

The obtained data will be analysed along with the dry lab team to determine the ordering of the enzymes in our plasmid for optimal functioning of our system