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Description

Paired-end FASTQ files were then run through the DADA2 pipeline (v1.8) to generate an amplicon sequence variant (ASV) table and assign taxonomy

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Within MSFraggers, precursor mass tolerance was set to +/-20ppm, fragment mass tolerance was set to +/-20ppm, tryptic cleavage specificity was applied, as well as fixed methionine oxidation and fixed N-terminal acetylation