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The GST-Trx2 fusion protein was purified by elution with a buffer containing 1XPBS, 5 mM DTT and 20 mM reduced glutathione on AKTA automated protein purifier (GE Biosciences)

The reader should be set to "kinetic mode" at 340 nm

Some boutique wellness centers advertise these injections as part of beauty regimens that claim to promote clearer, more radiant skin