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Vitamin E treatment in NAFLD patients demonstrates that oxidative stress drives steatosis through upregulation of de-novo lipogenesis

The membrane was blocked with 5% dry skim milk in Tris-buffer saline (TBS) containing 0.05% Tween-20 (TBS-T), incubated with primary rabbit anti-GST polyclonal antibody (1:10,000) or primary mouse anti-His polyclonal antibody (1:10,000) (Proteintech Group, Chicago, USA), and then incubated with secondary alkaline phosphatase conjugated goat anti-rabbit or anti-mouse antibody (1:2,000) (Proteintech Group, Chicago, USA)

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For storage and handling, always follow the instructions provided by your pharmacy