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Outliers were removed from control data after identification with ROUT Q = 0.5% (number of outliers removed: NAD + /NADH ratio n = 2, NADP + /NADPH ratio n = 11 and GSH/SSG ratio n = 6, no outliers were identified for individual metabolites)

Therefore, the intracellular GSH concentration is proposed as a potential molecular marker for cisplatin resistance in glioma, and the use of GSH inhibitors, such as BSO, in association with cisplatin and TMZ seems a promising approach for the therapy of this tumor

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The metabolism of glucose, fats, and amino acids produces ATP, which is required for cellular energy processes