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Briefly, oligonucleotides targeting Vgf were annealed and cloned into the Pshuttle-U6-CMV-GFP vector at the Sal I and Xba I sites

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Biochem Biophys Res Commun 417:4348 Shukla D, Mandal PK, Tripathi M, Vishwakarma G, Mishra R, Sandal K (2020) Quantitation of in vivo brain glutathione conformers in cingulate cortex among age-matched control, MCI, and AD patients using MEGA-PRESS