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Mitochondrial stress may increase free-radical production, while oxidative damage may further impair the electron transport chain

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qRT-PCR was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, United States) using FastStart universal SYBR Green Master reagent (ROX, Roche, Basel, Switzerland)

The genetic defects range from rare gene deletion and mutations to single nucleotide polymorphisms with more mild and subtle effects