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While information was found only from one study with 31 mood episodes of 26 patients with bipolar disorder, and 18 controls, it seems that while a depressive phase is associated with a phase delay, a manic phase is associated with a phase advance [53]

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The fractions containing the complex were pooled and concentrated using an Amicon Ultra concentrator with a 100 kDa cut-off (Millipore) to ~5 mg/ml

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