glutathione and glutamate cysteine liaase metabolic circuitries: druggable targets in cancer Glutathione synthesis. GSH synthesis relies

Elevated ABA levels inhibit ascorbate biosynthesis which in turn suppresses GA levels (Ye et al., 2012) while in contrast, ROS levels are reported to induce biosynthesis of GA as in Arabidopsis through enhanced gene expression (Liu et al., 2010)

doi: 10.3389/fcimb.2022.997018 42 FengY.WangY.WangP.HuangY.WangF

Tissue pieces were then transferred to 5 mL tubes (Eppendorf, #0030119487) containing 2 mL of cold HBSS 1 supplemented with 10% Fetal Bovine Serum (Corning, #35-010-CV) and centrifuged for 5 min at 4 C at 500 g and supernatant was pipetted out and discarded

93 Unlike other GPX4 inhibitors, ML210 is a prodrug that within cells is converted to the active form -nitroketoxime JKE1674 that covalently inhibits GPX4