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The coverslips containing the cells were then washed with ice-cold PBS three times, fixed with 4% paraformaldehyde at 4 C for 30 min, permeabilized with Triton X-100 for 10 min, and subjected to three 5-min washes

Drug candidates 110 were obtained from ASINEX (Gold Platinum library: BAS00402444, BAS00295409, BAS01402908, BAS00434324, BAS01293461, BAS05594113, BAS00512792, ASN08959436, ASN15408181, BAS03450598)

From an experimental design standpoint, sample sizes are imbalanced across time points, due to the sacrifice of a subset of animals at each time point for histological analysis

[20] in a comprehensive meta-analysis brought up a 52.4% decline in sperm concentration among men from North America, Europe, Australia, and New Zealand between 1973 and 2011, indicating the growing concern of a global decline in sperm quality and quantity, further underscoring the urgent need for interventions to improve male infertility