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Subsequently, samples were washed 3 times with PBS, followed by incubation with goat anti-rabbit IgG (H + L) Alexa Fluor 647 secondary antibody (ab190589, 1/100, Abcam) or goat anti-rabbit IgG (H + L) Alexa Fluor 488 secondary antibody (ab281847, 1/100, Abcam) for 3 hours, and then washed 3 times with PBS again

Aluminum inhibits proteolytic degradation of amyloid beta peptide by cathepsin D: a potential link between aluminum accumulation and neuritic plaque deposition

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the composition preferably includes ascorbic acid that helps to maintain the GSH in a reduced state, maintains a somewhat acidic environment in the mouth to avoid deprotonation of the glutamic acid residue, without causing substantially all of the amines to be protonated