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Human studies Most of the human experiments were carried out using LC as a supplement to alleviate/combat the problem of female infertility [11, 18, 36] (Table 1)

The exclusion criteria were as follows: (1) admitted for medical issues other than epilepsy, (2) pregnancy or planning to become pregnant, (3) history of milk allergy or lactose intolerance, (4) contraindications to KD such as diagnosed metabolic diseases, (5) diagnosis of a progressive neurological disorder

doi: 10.1080/1745039X.2017.1421340 28 TamaiIOhashiRNezuJYabuuchiHOkuAShimaneMet al

The cell debris was removed by centrifugation (45 min, 28,000 g , 4C), and the extracts were stored on ice until use in the enzyme assay and for determination of protein concentration with Bradford Reagent (Sigma-Aldrich, St