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If youre preparing your own syringes in advance, draw only what youll use within one week and store them upright in a sealed container to minimise air exposure

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Quantitative real-time PCR (qPCR) was performed with either 2.5 L diluted cDNA (12.5 ng RNA) mixed in a 10 L Kapa SYBR FAST qPCR Master Mix Universal (KapaBiosystems, Wilmington, MA, US), or 9 L diluted cDNA (25 ng RNA) and 1 L predeveloped TaqMan Gene Expression Assays were mixed in 10 L TaqMan Gene Expression Master Mix (Thermo Fisher Scientific, Waltham, MA, US), on a Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, US)

The marked effects of IGF-1 on onset and survival were accompanied by preserved morphology of motoneurons