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Serum was collected from the rabbits on day 70 and underwent purification through positive selection for affinity to the phosphopeptide, followed by negative selection to ensure no affinity to the non-phosphorylated peptide, CLEKTDLDSGGFGKVS-amide

Library quality was evaluated with a Bioanalyzer 2100 (Agilent), and sequencing was performed on the Illumina X-ten platform using paired-end 150 bp reads

Melanotan II Purported Benefits: Skin tanning without UV exposure Potential libido enhancement Potential Risks and Complications: Increased Risk of Melanoma: Stimulating melanin production can promote the development of skin cancers

GH deficiency In principle, the renal changes observed in patients with GH-deficiency mirror those observed in patients with GH-oversecretion