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The water-soluble nature of B12 means several things for patients : the vitamin requires regular dietary intake to maintain optimal levels, it can be lost during food preparation (particularly with prolonged cooking or boiling), and therapeutic doses are generally considered safe as excess is eliminated through the kidneys, though the body's substantial hepatic storage also plays a role in B12 metabolism

Acknowledgements This work was supported under grants from the National Natural Science Foundation of China (grant numbers: 22076104, 91943301, 21920102007, and 81970427), the International Collaboration Key Grant from the Chinese Academy of Sciences (grant number: 121311KYSB20190010), the Outstanding University Driven by Talents Program and Academic Promotion Program of Shandong First Medical University (grant number: 2020LJ002), and National Key R&D Program of China (grant number: 2021YFE0101500)

Theranostic nanosystem mediating cascade catalytic reactions for effective immunotherapy of highly immunosuppressive and poorly penetrable pancreatic tumor