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After treatment of the cells with AuNPs (700 and 70 g/mL in a final volume of 1 mL) for 1 h and 2 h, Hep G2 cells were fixed as a monolayer in 2% glutaraldehyde in cacodylate buffer 0.1 mol/L pH 7.4 for 30 min at room temperature, then scraped and pelleted

2 Revisiting bacteria biofilm ultrastructural and its antibiotic survival strategies Bacterial biofilms are groups of sessile microbes that are entrenched and connected to substratum, within the self-generated non-crystalline pool of the extracellular matrix (Zuberi et al., 2017a, Zuberi et al., 2017b)

2.4 Cell lines and culture conditions The RAW 264.7 (ATCC TIB-61) and A549 (human lung epithelial carcinoma, pneumocytes type II