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Ethical approval Ethical approval for these studies was obtained from the University of Central Lancashires Faculty of Health Research Ethics Committee which accepted that the study had been granted exemption by the Department of Healths National Information Governance Board from the need to obtain informed consent from individuals retrospectively to participate in the mortality study

After the elevated T-maze test, the locomotor activity was evaluated in the open field test in order to validate and confirm the behavioural responses seen in the apparatus 38

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Researchers reported changes in the expression levels of 123 genes when reconstituted lung tissue was exposed to cigarette smoke, compared to only two genes that could be confirmed following exposure to e-cigarette aerosols, according to a press release