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Sajjad H, Alam F, Dalvi F, Durrani N

Consult a doctor if you experience: Persistent fatigue that doesnt improve with rest Breathlessness during routine activities Frequent dizziness or fainting Heavy menstrual bleeding (more than 7 days or soaking pads rapidly) Blood test results showing hemoglobin below 10 g/dL Symptoms that are not improving despite two to three months of dietary changes Chest pain, severe weakness, or sudden fainting require emergency medical attention immediately

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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)