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Thermostability ( T 50 ) The kinetic thermostability of purified LacAnc100 and LaccID was estimated by assessing their T 50 values using 96-well gradient thermocyclers (T100 thermal cycler, Bio-Rad)

For decades, research has centered on glucose and lipid metabolism disorders (e.g., insulin resistance and hyperlipidemia) as central drivers of metabolic diseases ( As an essential AAA, phenylalanine (Phe) metabolism is predominantly governed by hepatic phenylalanine hydroxylase (PAH) ( Interestingly, gut microbiota-derived Phe metabolites, such as phenylacetylglutamine (PAGln) and exercise-induced N-lactoyl-phenylalanine (Lac-Phe), demonstrate diverse and beneficial bioactivities in cardiovascular risk modulation and appetite regulation, respectively ( Therefore, understanding the mechanisms by which dysregulated Phe triggers metabolic dysfunction is crucial

Anti-Inflammatory Potential of 1-Nitro-2-Phenylethylene

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