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Calibration against 100 mM H 2 O 2 to fully oxidize and 10 mM DTT to fully reduce the sensor (Figure 1A and B) and an assumed cytosolic pH of 7.4 led to an estimation of the glutathione redox potential (E GSH ) of - 309mV +/-10 at 20C in palissadic cotyledon cells and to a less negative E GSH to 247 mV +/-21 after 1 hour treatment at 37C