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After 18 h of viral infection, media was replaced with fresh NPC media supplemented with 2.5 g/ml of doxycycline (STEMCELL Technologies, Vancouver, Canada 72742) and 20 ng/ml bFGF (R&D systems 4114-TC-01M) (Day 0)

Stromal adaptations in tumor-draining LNs The LN stromal cells provide a framework structure that maintains the niches for immune cells, acts as a backbone for immune cell migration, and forms a conduit system to filter antigens in the lymph (122125)

5-Amino-1MQ blocks the NNMT enzyme, shifting fat-cell energy balance and raising intracellular NAD+, a target validated by a 2014 Nature mouse study [2]

In sum, we demonstrate that repRNA/LION delivery of gH/gL elicited high titers of neutralizing antibodies that protected against lethal challenge in a humanized mouse infection model