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The expression of mitochondrial methylenetetrahydrofolate dehydrogenase 2 (MTHFD2), which is necessary for purine synthesis, depends on ATF4

The NO release capability of HMO was further assessed using the Griess reagent method

In particular, miR-16 was reported to be downregulated in osteosarcoma cell lines and tissues (77)

Glutathione S-transferase (GST) activity was assessed by adding 10 L of the tissue supernatant to a mixture of 10 L of GSH (5 mM) and 10 L of 2,4-dinitrobenzene (CDNB) (1 mM) in 100 L of phosphate buffer, recording at 340 nm for 5 min, and the activity was expressed as moles of CDNB conjugated/min/mg of protein (Al Kury et al., 2019)