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The mainly chromosome-encoded response to chloromethane involves gene clusters associated with oxidative stress, production of reducing equivalents (PntAA, Nuo complex), conversion of tetrahydrofolate-bound one-carbon units, and central metabolism

At the later steps, after extract clean-up, pH of the sample should be controlled to avoid analyte loss, since some clean-up steps might increase sample pH and endanger the stability of base-sensitive compounds like patulin

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For example, GST plays a key role in regulating the MAP kinase pathway via protein: protein interactions