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The cell pellet was resuspended at room temperature for 10 min with Flow Cytometry Staining Buffer (Catalog #00-4222, eBioscience, Waltham, MA, USA) containing anti-mouse CD16/32 antibody and then stained with one of the following antibodies at room temperature: anti-mouse CD11b antibody conjugated with PE-Cyanine7 or anti-mouse GL3 antibody conjugated with PE