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These observations suggest a significant contribution of CYPOR to NFT-induced Nrf2 activation in the HepG2 model, but also indicate that other cellular flavoprotein reductases are likely to be involved in the formation of the NFT radical anion and its subsequent activation of Nrf2 and synthesis of GCLC and glutathione

After 24 h of incubation, the cells were incubated with 5 mg/mL MTT reagent (Sigma Aldrich, Germany) for 4 h at 37

These are called Guideline-Directed Medical Therapy or GDMT