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If the patient is critically ill, or has neurologic disease, an infectious disease or hyperthyroidism, considerably higher doses may be indicated

After centrifugation (14,000 rpm, 10 min, 4 C), the supernatant was removed, and the pellet was washed with acetone and dissolved in 10 L of SDS sample buffer (125 mM Tris-HCl, 4% SDS, 20% glycerol, 100 mM DTT, 0.01% bromophenol blue)

In addition, when SELENOW was placed on a human fetal brain cDNA library, it was identified to interact with FAM96B, which was verified by co-immunoprecipitation

doi: 10.1007/s10876-021-02036-1 231 ThatoiPKerryRGGoudaSDasGPramanikKThatoiHet al