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Rodriguez-Chavez V, Flores-Soto E, Molina-Salinas G, Martinez-Razo LD, Montano LM, Cerbon M

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Cells (1 10 6 ) were transduced with 0.51 ml of viral supernatant in 2 ml of growth medium with 10 mM HEPES and 8 g ml 1 polybrene in six-well format and centrifuged at 2,600 rpm for 2 h at 35 C

From our experience, these relate mainly to the following difficulties: (1) Clinical documents are variously structured, notational, lengthy, and incomplete