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Methotrexate is also given before treatment with leucovorin to prolong relapse-free survival following surgical removal of a tumour in non-metastatic osteosarcoma

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However, many studies have focused exclusively on proton magnetic resonance spectroscopy (1H-MRS) and have not incorporated markers of oxidative stress (OS) and neuroinflammation

Methods The study was designed with one screening round (9 doses over 3 days) of DMSA in Phase 1, followed by a randomized, double-blind, placebo-controlled study of 6 additional rounds (see Figure 1)