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174,175 In cases of vitamin B 12 depletion induced by HRA medications, levels of the vitamin have improved as a result of oral vitamin B 12 supplementation (as opposed to food source vitamin B 12 ingestion) as well as discontinuation of H2RA therapy

For GSH assay, cells were treated in Earles balanced salt solution (EBSS), supplemented with 25 mM glucose, 4 mM glutamine, 1 X MEM vitamin solution (Life technologies, CA, USA), 0.22% wt/vol NaHCO 3 , 1 mM sodium pyruvate, 0.2% BSA, 25 mM HEPES, 100 U/ml penicillin and 100 g/ml streptomycin in the presence or absence of 1 X MEM amino acid solution (Gibco, CA, USA) with or without 100 M cystine

Secondly, the freed-up vitamin B12 must combine with another protein (called the intrinsic factor) in the stomach so that the body can absorb it