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To do this, rats were sacrificed and the posterior gastrocnemius muscles on the injured and uninjured hind limbs were weighed and the wet weight-loss of gastrocnemius muscles was calculated by the following equation: 2.9.4 Functional assessment of sensory recovery (Hot plate latency test) Twelve weeks post-surgery, the rats were evaluated for hot plate latency (HPL) by placing their injured limbs on the center of a hot plate (56 C) and recording the time until they reacted (by jumping or licking their paws)