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Akt phosphorylation leads to the phosphorylation of glycogen synthase kinase-3(GSK3) inhibiting GSK3 activity and effecting on its substrates including Bax, NF-B, TNF-, IL-6, tau protein, and c-Jun transcript [91, 92]

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However, ultrasound alone is not recommended for routine screening due to lower sensitivity for microcalcifications and higher false-positive rates, which may lead to unnecessary biopsies and follow-up imaging

Acyltransferases mediating butyrylation exhibited distinct substrate specificities: in vitro studies demonstrated that CBP/p300 (CREB-binding protein/E1A binding protein p300) directly butyrylates both histone H4 and non-histone protein p53 [58], whereas Gcn5 mutation significantly reduced histone H3 butyrylation [60]