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The most commonly reported side effect in animal studies was mild local irritation at the injection site, which resolved without sequelae

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Some side effects of levodopa have been reported, including sleep, psychological disturbances, and gastrointestinal adverse events such as nausea and vomiting

Parasite pellets were resuspended in buffer (3.5 mM MgCl 2 , 110 mM KCl, 40 mM NaCl, 20 mM HEPES, 6 mM EDTA, pH 7.4) with protease inhibitors (0.01 mg of leupeptin A, 0.001 mg of pepstatin A, 0.35 mg of PMSF) lysed by three freeze/thaw cycles (liquid nitrogen and 37C water bath), and protein content was determined using Bio-Rad DC Protein Assay