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The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm

TNF- damages cardiomyocyte mitochondria by inhibiting glycolysis and reducing mitochondrial adenosine triphosphate (ATP) production through promoting oxidative and nitrosative stress, which can result in compromised cardiac systolic function and unfavorable cardiac remodeling [83, 84]

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During the past few decades, numerous research endeavors have been dedicated to elucidating the underlying mechanisms and identifying potential biomarkers of ALS