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Application frequency for topical protocols is typically once or twice daily, applied to cleansed skin at the target area

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Hair and nail germ formation and elongation assay For the formation and elongation assay of hair and nail germs, primary skin dermal fibroblasts, skin epidermal keratinocytes, hair follicle dermal papilla cells, and nail fibroblasts (onychofibroblasts) were obtained from patients under ethical considerations