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The cell apoptosis was detected by using a Flow cytometry (Beckman, Navios 2 L 8 C, USA)

Additionally, bovine mammary alveolar cells-large T antigen (MAC-T) cell line was used as positive control cells to validate the cytokeratin-18 staining (1:50/1:100)

doi: 10.1016/j.cmet.2012.04.001 386 RuanHPownallHJ

Research in the notes that an estimated 1025% of people who use benzos for extended periods experience withdrawal symptoms that last for 12 months or longer