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Long-term temperature exposures of fish result in upregulated antioxidants such as SOD, CAT, GPx, GR, and glutathione-S-transferase (GST), which are more apparent under heat stress than cold stress

28 Further, it transformed into water in the presence of catalytic enzymes like glutathione peroxidases (GPx) and peroxiredoxins

Additionally, the extravasation of immune cells, particularly neutrophils and monocytes, further amplifies the astrocyte activation process through the release of proinflammatory cytokines and chemokines (54)

Meantime, hepatic and renal function biochemical markers tests exhibited normal ranges of values in the PBS, Gi-F-CAA (8 mg/kg in 100 L PBS), Gi-F-CAA (12 mg/kg in 100 L PBS) and Gi-F-CAA (16 mg/kg in 100 L PBS) treatment groups at 1 day post-injection, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), urea nitrogen (BUN), and creatinine (CRE) levels (Supplementary Fig