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Weeks 16-52: Final phase The length of isotretinoin treatment varies depending on individual factors like body weight, dosage tolerance, and acne severity

Erk phosphorylation was assessed in 50 g HepG2 extracts run on 10% gels and transferred onto a PDVF membrane

Among the potential therapeutic approaches, interventions targeting endothelial dysfunction (such as RAAS modulators, and flavonoids) and persistent inflammation (e.g., antioxidants, vitamin C, vitamin D, and AMPK activators like metformin) appear particularly promising