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Briefly, 100-L of different concentration standards or diluted plasma samples (1:10) were added to the wells in duplicate, and the plate was incubated for 60 min at 37 C

Similarly, in in vivo experiments, Lip-1, a ferroptosis inhibitor, decreased neuronal death and neuro-inflammation in EBI by protecting mitochondrial functions and reducing lipid peroxidation, thereby ameliorating cerebral edema and neurological deficits after SAH ( 3 The Mechanism of Ferroptosis Studies have indicated that ferroptosis may be induced by different physiological conditions and pathological stress

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