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For HE staining, tissue sections were deparaffinized in xylene I and II for 10 min each, then sequentially hydrated for 5 min each in xylene/ethanol (1:1), 100% ethanol I/II and 80% ethanol, followed by rinsing with tap water

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However, microdialysis of the pVTA shows that salsolinol is not entirely absent after alcohol administration with DPCPX, although not significantly different from baseline (Fig