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The PCR products were purified with a QIA quick PCR purification column (Qiagen, GmbH, Hilden, Germany) following the manufacturers instructions and then sequenced with an ABI 3730XL DNA sequencer (Biosystems, USA)

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Whether the end products of AS-IV after in vivo metabolism are also involved in the derivation of AS-IV's antirenal fibrosis activity requires a comprehensive mechanism integration analysis

A substantial body of research conducted in animals has consistently demonstrated that the disruption of the normal light-dark cycle may significantly increase cancer incidence, tumor growth, and progression 40,41