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Cellular GSH is found mostly in the cytosol, with the remainder spread across organelles ( The thioredoxin system is composed of Trx proteins and Trx reductases (TrxR), in which the reduced form of Trx proteins (Trx(SH) 2 ) are disulfide reductases as they contain dithiol groups in their highly conserved active site (-Cys-Gly-Pro-Cys-) ( 2 can directly reduce oxidized proteins or provide electrons to thiol-dependent peroxidases (Prx) to convert hydrogen peroxide to water

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10.1104/pp.58.5.700 Plant Physiol

For glutathionylation experiments, 30 10 6 cells were washed with RPMI supplemented with 0.5% FBS and 100 U/mL penicillin-streptomycin and resuspended in a total volume of 5 mL of the same medium