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Hypothyroidism is classified as primary or secondary, with primary causes including iodine deficiency, autoimmune diseases (e.g., Hashimotos thyroiditis), enzymatic damage, radiotherapy, thyroid surgery, certain medications, and chronic or infiltrative conditions, while secondary hypothyroidism results from pituitary or hypothalamic disorders

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To construct the modified homozygous strain expressing GAP1 as the counter-selectable marker in the BY4743 background, the KanMX fragment was amplified from pFA6A-KanMX6 [32], and HS fragments for pGAP1 were amplified from yeast genomic DNA and these fragments plus pGSH1 (above) assembled into pRS315 to give pRS315- HS-KanMX-pGSH1-HS

Myeloid cells, including macrophages and neutrophils, are especially implicated in the respiratory burst