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Cells were grown in LB medium with ampicillin, induced with 0.5 mM IPTG at 25 C for 1012 h, and harvested after cooling to 4 C

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To monitor the possibility of mycoplasma infection in the mouse skin tissues, the levels of mycoplasma pathogens were detected in a variety of tissue lysate (e.g., whole cell lysate, nuclear extract) using a MycoAlert Mycoplasma Detection Kit purchased from Lonza (Rockland, ME), and the results were negative