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To assess whether the observed reduction in freezing response was due to altered pain sensitivity, mice were subjected to the hot plate test

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SCFA measurement using LCMS Serum (5 l) or intestinal content (1 mg) was mixed with derivatizing reagent (100 l) and incubated for 1 h at 4 C

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However, among the members of the Neoaves clade, proportion of the species with a long lifespan is not very high [72]

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