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The functional activity of FVII from supernatants of iPSC-HO was assessed by two methods: a) western blotting after activation with tissue factor (TF, STA Neoplastin) and CaCl 2 and incubation with antithrombin (AT), purified from human plasma, (Grifols, Spain) and unfractionated heparin (Hospira) to measure the ability of FVII to become active and form a complex with AT

When the body is under excessive stress, these proteins exert a protective role by lessening oxidative action of the reactive oxygen species (ROS) and a wide range of metabolic stress, including structural and functional myodamage [87, 88]

Vitamin B12 absorption depends on a protein produced by the stomach called intrinsic factor

The mean MELD at baseline is 11.3