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We assume the value of E Cl to be such that Cl concentration is at equilibrium, that is the Cl synaptic influx and extrusion through KCC2 cancel each other out, this yields where V mean is the time averaged value the membrane potential taken to be V mean = 60 mV

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For global splicing analyses, we only considered the 6222 major isoforms of protein-coding genes with a prevalence higher than 70%, no overlap with other genes, and with read counts per kilobase (RPK) higher than 50, as previously described 8,73

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