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Colonies with Hif1a KO were selected by flow cytometry single cell sorting and expression validated by immunoblotting

At 60 h-post injection, the mice were sacrificed and the biodistribution determined upon fluorescence imaging of the respective organs

We carefully reviewed canonical marker gene expression on UMAP plots and repeated the steps above a couple of times to ensure that we had filtered out most of the barcodes associated with cell doublets

Immune and Recovery Support Certain nutrients delivered intravenously may support immune function and aid the bodys natural recovery processes by contributing to cellular repair and reducing oxidative stress