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2.9 Proteomic and mass spectrometry analysis Mtb supernatants were prepared as above described, concentrated by ultrafiltration then denatured and electrophoresed by 4%15% polyacrylamide gel (SDS-PAGE) and stained with Coomassie, according to published protocols with few modifications (Verdoliva et al., 2013)

doi:10.1073/pnas.1000138107

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By acting as a carrier and regulator of bioavailable copper, GHK-Cu plays a role in maintaining metal homeostasis and influencing downstream pathways linked to cellular growth, differentiation, and tissue remodelling