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The aim of this study was to clarify the relationship between NLR and bleeding or NLR and prognosis, after endoscopic treatment for esophageal varices in cirrhotic patients with esophageal varices

Liver histological staining and immunolabelling Pieces of liver tissue from postmortem PBS-perfused mice (at least 3 animals per group) were fixed in 4% paraformaldehyde for 12 h, stored in 70% ethanol, and embedded in paraffin blocks and sectioned using an RM2165 microtome (Leica Microsystems)

For cytokine and chemokines measurements, Sybr Green PCR Master Mix (Applied Biosystems) was used to carry out the reactions in an QuantStudio 12 K Flex Real-Time PCR System (Thermo Fisher Scientific) under standard conditions

Lipotropic Injections Containing Vitamin B12 Lipo shots contain lipotropes a mixture of ingredients, such as vitamin B12, amino acids, etc