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The unbound antibody was washed away, followed by further incubation of the membranes with horseradish peroxidase (HRP)-conjugated rabbit secondary antibodies (Beyotime Biotechnology, Jiangsu, China) at room temperature for 1 h

Such a diet often increases Akkermansia in the gut (since Akkermansia thrives on mucin when fiber is low and fats are high), which as mentioned might produce nicotinamide and other beneficial compounds [211]